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facs tubes sarstedt  (Nikon)


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    Nikon facs tubes sarstedt
    Facs Tubes Sarstedt, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+tubes+sarstedt/Tubes/pm41838718-62-259-265
    Average 96 stars, based on 704 article reviews
    facs tubes sarstedt - by Bioz Stars, 2026-09
    96/100 stars

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    Recombinant:

    Article Title: Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imaging.
    Article Snippet: MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) Gibco Cat#A1443001 2-Deoxy-D-Glucose (2-DG) Carl Roth Cat#CN96.2 Adenosine triphosphate (ATP) Carl Roth Cat#HN35.2 Cal-520 AM AAT Bioquest Cat#21130 Calcium chloride dihydrate (CaCl 2 ), CellPure Carl Roth Cat#HN04.2 DPBS, no calcium, no magnesium Gibco Cat#14190094 Fetal Bovine Serum (FBS) PAN-Biotech Cat#P30-3302 Fluo-3 AM AAT Bioquest Cat#21011 Fura Red AM AAT Bioquest Cat#21048 Glucose (stock 1.1 M) Gibco Cat#A2494001 HBSS, w/Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-32505 HBSS, w/o Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-34500 IMDM (w/5.5 mM Glucose, w/o L-Glutamine) PAN-Biotech Cat#P04-20157 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldirch Cat#I0634-1MG L-Glutamine (stock 200 mM) PAN-Biotech Cat#P04-80100 Meso-Erythritol Thermo Scientific Car#A15813-14 Penicillin/Streptomycin PAN-Biotech Cat#P06-07100 Recombinant murine MCP-1 (CCL2) ImmunoTools Cat#12343383 Recombinant murine MIP-3β (CCL19) PeproTech Cat#250-27B Sodium Pyruvate (100 mM) PAN-Biotech Cat#P04-43100 Sucralose Biomol Cat#Cay31621-25 Thapsigargin Biomol Cat#Cay10522-1 UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Cat#15575020 β-Mercaptoethanol Carl Roth Cat#4227.3 Experimental models: Cell lines Mouse: J558L GM-CSF producing line Lutz et al., 7 , Burgdorf et al. 8 N/A Experimental models: Organisms/strain Mouse: C57BL76 (age: 8–12 weeks, male or female) Own breeding, N/A Software and algorithms BD FACS Diva BD Biosciences N/A Excel/Word, Office Standard 2019 Microsoft Office N/A FlowJo, Version 10 BD Biosciences N/A Graphpad Prism, Version 10 GraphPad Software https://www.graphpad.com/ Image J Schneider et al. 9 https://imagej.net/ij/ Other 15 mL/50 mL conical tubes Sarstedt Cat#62.554.502 BD LSR II, Flow Cytometer BD Biosciences N/A BD Symphony A5 BD Biosciences N/A Cell imaging plate, flat coverglass bottom Eppendorf Cat#0030741021 FACS tubes Sarstedt Cat#55.1579 Brightfield microscope: Nikon TS1000, Epifluorescence Microscopes: Nikon TI2, Olympus IX81 Nikon, Olympus N/A Medium containing physiological levels of glucose, pyruvate and glutamine Reagent Final concentration Amount DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) N/A 500 mL FBS 10% 50 mL Penicillin/Streptomycin 1% 5 mL (Continued on next page) 4 STAR Protocols 7, 104435, June 19, 2026

    Software:

    Article Title: Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imaging.
    Article Snippet: MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) Gibco Cat#A1443001 2-Deoxy-D-Glucose (2-DG) Carl Roth Cat#CN96.2 Adenosine triphosphate (ATP) Carl Roth Cat#HN35.2 Cal-520 AM AAT Bioquest Cat#21130 Calcium chloride dihydrate (CaCl 2 ), CellPure Carl Roth Cat#HN04.2 DPBS, no calcium, no magnesium Gibco Cat#14190094 Fetal Bovine Serum (FBS) PAN-Biotech Cat#P30-3302 Fluo-3 AM AAT Bioquest Cat#21011 Fura Red AM AAT Bioquest Cat#21048 Glucose (stock 1.1 M) Gibco Cat#A2494001 HBSS, w/Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-32505 HBSS, w/o Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-34500 IMDM (w/5.5 mM Glucose, w/o L-Glutamine) PAN-Biotech Cat#P04-20157 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldirch Cat#I0634-1MG L-Glutamine (stock 200 mM) PAN-Biotech Cat#P04-80100 Meso-Erythritol Thermo Scientific Car#A15813-14 Penicillin/Streptomycin PAN-Biotech Cat#P06-07100 Recombinant murine MCP-1 (CCL2) ImmunoTools Cat#12343383 Recombinant murine MIP-3β (CCL19) PeproTech Cat#250-27B Sodium Pyruvate (100 mM) PAN-Biotech Cat#P04-43100 Sucralose Biomol Cat#Cay31621-25 Thapsigargin Biomol Cat#Cay10522-1 UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Cat#15575020 β-Mercaptoethanol Carl Roth Cat#4227.3 Experimental models: Cell lines Mouse: J558L GM-CSF producing line Lutz et al., 7 , Burgdorf et al. 8 N/A Experimental models: Organisms/strain Mouse: C57BL76 (age: 8–12 weeks, male or female) Own breeding, N/A Software and algorithms BD FACS Diva BD Biosciences N/A Excel/Word, Office Standard 2019 Microsoft Office N/A FlowJo, Version 10 BD Biosciences N/A Graphpad Prism, Version 10 GraphPad Software https://www.graphpad.com/ Image J Schneider et al. 9 https://imagej.net/ij/ Other 15 mL/50 mL conical tubes Sarstedt Cat#62.554.502 BD LSR II, Flow Cytometer BD Biosciences N/A BD Symphony A5 BD Biosciences N/A Cell imaging plate, flat coverglass bottom Eppendorf Cat#0030741021 FACS tubes Sarstedt Cat#55.1579 Brightfield microscope: Nikon TS1000, Epifluorescence Microscopes: Nikon TI2, Olympus IX81 Nikon, Olympus N/A Medium containing physiological levels of glucose, pyruvate and glutamine Reagent Final concentration Amount DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) N/A 500 mL FBS 10% 50 mL Penicillin/Streptomycin 1% 5 mL (Continued on next page) 4 STAR Protocols 7, 104435, June 19, 2026

    FACS:

    Article Title: Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imaging.
    Article Snippet: MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) Gibco Cat#A1443001 2-Deoxy-D-Glucose (2-DG) Carl Roth Cat#CN96.2 Adenosine triphosphate (ATP) Carl Roth Cat#HN35.2 Cal-520 AM AAT Bioquest Cat#21130 Calcium chloride dihydrate (CaCl 2 ), CellPure Carl Roth Cat#HN04.2 DPBS, no calcium, no magnesium Gibco Cat#14190094 Fetal Bovine Serum (FBS) PAN-Biotech Cat#P30-3302 Fluo-3 AM AAT Bioquest Cat#21011 Fura Red AM AAT Bioquest Cat#21048 Glucose (stock 1.1 M) Gibco Cat#A2494001 HBSS, w/Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-32505 HBSS, w/o Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-34500 IMDM (w/5.5 mM Glucose, w/o L-Glutamine) PAN-Biotech Cat#P04-20157 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldirch Cat#I0634-1MG L-Glutamine (stock 200 mM) PAN-Biotech Cat#P04-80100 Meso-Erythritol Thermo Scientific Car#A15813-14 Penicillin/Streptomycin PAN-Biotech Cat#P06-07100 Recombinant murine MCP-1 (CCL2) ImmunoTools Cat#12343383 Recombinant murine MIP-3β (CCL19) PeproTech Cat#250-27B Sodium Pyruvate (100 mM) PAN-Biotech Cat#P04-43100 Sucralose Biomol Cat#Cay31621-25 Thapsigargin Biomol Cat#Cay10522-1 UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Cat#15575020 β-Mercaptoethanol Carl Roth Cat#4227.3 Experimental models: Cell lines Mouse: J558L GM-CSF producing line Lutz et al., 7 , Burgdorf et al. 8 N/A Experimental models: Organisms/strain Mouse: C57BL76 (age: 8–12 weeks, male or female) Own breeding, N/A Software and algorithms BD FACS Diva BD Biosciences N/A Excel/Word, Office Standard 2019 Microsoft Office N/A FlowJo, Version 10 BD Biosciences N/A Graphpad Prism, Version 10 GraphPad Software https://www.graphpad.com/ Image J Schneider et al. 9 https://imagej.net/ij/ Other 15 mL/50 mL conical tubes Sarstedt Cat#62.554.502 BD LSR II, Flow Cytometer BD Biosciences N/A BD Symphony A5 BD Biosciences N/A Cell imaging plate, flat coverglass bottom Eppendorf Cat#0030741021 FACS tubes Sarstedt Cat#55.1579 Brightfield microscope: Nikon TS1000, Epifluorescence Microscopes: Nikon TI2, Olympus IX81 Nikon, Olympus N/A Medium containing physiological levels of glucose, pyruvate and glutamine Reagent Final concentration Amount DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) N/A 500 mL FBS 10% 50 mL Penicillin/Streptomycin 1% 5 mL (Continued on next page) 4 STAR Protocols 7, 104435, June 19, 2026

    Flow Cytometry:

    Article Title: Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imaging.
    Article Snippet: MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) Gibco Cat#A1443001 2-Deoxy-D-Glucose (2-DG) Carl Roth Cat#CN96.2 Adenosine triphosphate (ATP) Carl Roth Cat#HN35.2 Cal-520 AM AAT Bioquest Cat#21130 Calcium chloride dihydrate (CaCl 2 ), CellPure Carl Roth Cat#HN04.2 DPBS, no calcium, no magnesium Gibco Cat#14190094 Fetal Bovine Serum (FBS) PAN-Biotech Cat#P30-3302 Fluo-3 AM AAT Bioquest Cat#21011 Fura Red AM AAT Bioquest Cat#21048 Glucose (stock 1.1 M) Gibco Cat#A2494001 HBSS, w/Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-32505 HBSS, w/o Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-34500 IMDM (w/5.5 mM Glucose, w/o L-Glutamine) PAN-Biotech Cat#P04-20157 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldirch Cat#I0634-1MG L-Glutamine (stock 200 mM) PAN-Biotech Cat#P04-80100 Meso-Erythritol Thermo Scientific Car#A15813-14 Penicillin/Streptomycin PAN-Biotech Cat#P06-07100 Recombinant murine MCP-1 (CCL2) ImmunoTools Cat#12343383 Recombinant murine MIP-3β (CCL19) PeproTech Cat#250-27B Sodium Pyruvate (100 mM) PAN-Biotech Cat#P04-43100 Sucralose Biomol Cat#Cay31621-25 Thapsigargin Biomol Cat#Cay10522-1 UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Cat#15575020 β-Mercaptoethanol Carl Roth Cat#4227.3 Experimental models: Cell lines Mouse: J558L GM-CSF producing line Lutz et al., 7 , Burgdorf et al. 8 N/A Experimental models: Organisms/strain Mouse: C57BL76 (age: 8–12 weeks, male or female) Own breeding, N/A Software and algorithms BD FACS Diva BD Biosciences N/A Excel/Word, Office Standard 2019 Microsoft Office N/A FlowJo, Version 10 BD Biosciences N/A Graphpad Prism, Version 10 GraphPad Software https://www.graphpad.com/ Image J Schneider et al. 9 https://imagej.net/ij/ Other 15 mL/50 mL conical tubes Sarstedt Cat#62.554.502 BD LSR II, Flow Cytometer BD Biosciences N/A BD Symphony A5 BD Biosciences N/A Cell imaging plate, flat coverglass bottom Eppendorf Cat#0030741021 FACS tubes Sarstedt Cat#55.1579 Brightfield microscope: Nikon TS1000, Epifluorescence Microscopes: Nikon TI2, Olympus IX81 Nikon, Olympus N/A Medium containing physiological levels of glucose, pyruvate and glutamine Reagent Final concentration Amount DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) N/A 500 mL FBS 10% 50 mL Penicillin/Streptomycin 1% 5 mL (Continued on next page) 4 STAR Protocols 7, 104435, June 19, 2026

    Imaging:

    Article Title: Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imaging.
    Article Snippet: MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) Gibco Cat#A1443001 2-Deoxy-D-Glucose (2-DG) Carl Roth Cat#CN96.2 Adenosine triphosphate (ATP) Carl Roth Cat#HN35.2 Cal-520 AM AAT Bioquest Cat#21130 Calcium chloride dihydrate (CaCl 2 ), CellPure Carl Roth Cat#HN04.2 DPBS, no calcium, no magnesium Gibco Cat#14190094 Fetal Bovine Serum (FBS) PAN-Biotech Cat#P30-3302 Fluo-3 AM AAT Bioquest Cat#21011 Fura Red AM AAT Bioquest Cat#21048 Glucose (stock 1.1 M) Gibco Cat#A2494001 HBSS, w/Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-32505 HBSS, w/o Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-34500 IMDM (w/5.5 mM Glucose, w/o L-Glutamine) PAN-Biotech Cat#P04-20157 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldirch Cat#I0634-1MG L-Glutamine (stock 200 mM) PAN-Biotech Cat#P04-80100 Meso-Erythritol Thermo Scientific Car#A15813-14 Penicillin/Streptomycin PAN-Biotech Cat#P06-07100 Recombinant murine MCP-1 (CCL2) ImmunoTools Cat#12343383 Recombinant murine MIP-3β (CCL19) PeproTech Cat#250-27B Sodium Pyruvate (100 mM) PAN-Biotech Cat#P04-43100 Sucralose Biomol Cat#Cay31621-25 Thapsigargin Biomol Cat#Cay10522-1 UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Cat#15575020 β-Mercaptoethanol Carl Roth Cat#4227.3 Experimental models: Cell lines Mouse: J558L GM-CSF producing line Lutz et al., 7 , Burgdorf et al. 8 N/A Experimental models: Organisms/strain Mouse: C57BL76 (age: 8–12 weeks, male or female) Own breeding, N/A Software and algorithms BD FACS Diva BD Biosciences N/A Excel/Word, Office Standard 2019 Microsoft Office N/A FlowJo, Version 10 BD Biosciences N/A Graphpad Prism, Version 10 GraphPad Software https://www.graphpad.com/ Image J Schneider et al. 9 https://imagej.net/ij/ Other 15 mL/50 mL conical tubes Sarstedt Cat#62.554.502 BD LSR II, Flow Cytometer BD Biosciences N/A BD Symphony A5 BD Biosciences N/A Cell imaging plate, flat coverglass bottom Eppendorf Cat#0030741021 FACS tubes Sarstedt Cat#55.1579 Brightfield microscope: Nikon TS1000, Epifluorescence Microscopes: Nikon TI2, Olympus IX81 Nikon, Olympus N/A Medium containing physiological levels of glucose, pyruvate and glutamine Reagent Final concentration Amount DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) N/A 500 mL FBS 10% 50 mL Penicillin/Streptomycin 1% 5 mL (Continued on next page) 4 STAR Protocols 7, 104435, June 19, 2026

    Microscopy:

    Article Title: Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imaging.
    Article Snippet: MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) Gibco Cat#A1443001 2-Deoxy-D-Glucose (2-DG) Carl Roth Cat#CN96.2 Adenosine triphosphate (ATP) Carl Roth Cat#HN35.2 Cal-520 AM AAT Bioquest Cat#21130 Calcium chloride dihydrate (CaCl 2 ), CellPure Carl Roth Cat#HN04.2 DPBS, no calcium, no magnesium Gibco Cat#14190094 Fetal Bovine Serum (FBS) PAN-Biotech Cat#P30-3302 Fluo-3 AM AAT Bioquest Cat#21011 Fura Red AM AAT Bioquest Cat#21048 Glucose (stock 1.1 M) Gibco Cat#A2494001 HBSS, w/Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-32505 HBSS, w/o Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-34500 IMDM (w/5.5 mM Glucose, w/o L-Glutamine) PAN-Biotech Cat#P04-20157 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldirch Cat#I0634-1MG L-Glutamine (stock 200 mM) PAN-Biotech Cat#P04-80100 Meso-Erythritol Thermo Scientific Car#A15813-14 Penicillin/Streptomycin PAN-Biotech Cat#P06-07100 Recombinant murine MCP-1 (CCL2) ImmunoTools Cat#12343383 Recombinant murine MIP-3β (CCL19) PeproTech Cat#250-27B Sodium Pyruvate (100 mM) PAN-Biotech Cat#P04-43100 Sucralose Biomol Cat#Cay31621-25 Thapsigargin Biomol Cat#Cay10522-1 UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Cat#15575020 β-Mercaptoethanol Carl Roth Cat#4227.3 Experimental models: Cell lines Mouse: J558L GM-CSF producing line Lutz et al., 7 , Burgdorf et al. 8 N/A Experimental models: Organisms/strain Mouse: C57BL76 (age: 8–12 weeks, male or female) Own breeding, N/A Software and algorithms BD FACS Diva BD Biosciences N/A Excel/Word, Office Standard 2019 Microsoft Office N/A FlowJo, Version 10 BD Biosciences N/A Graphpad Prism, Version 10 GraphPad Software https://www.graphpad.com/ Image J Schneider et al. 9 https://imagej.net/ij/ Other 15 mL/50 mL conical tubes Sarstedt Cat#62.554.502 BD LSR II, Flow Cytometer BD Biosciences N/A BD Symphony A5 BD Biosciences N/A Cell imaging plate, flat coverglass bottom Eppendorf Cat#0030741021 FACS tubes Sarstedt Cat#55.1579 Brightfield microscope: Nikon TS1000, Epifluorescence Microscopes: Nikon TI2, Olympus IX81 Nikon, Olympus N/A Medium containing physiological levels of glucose, pyruvate and glutamine Reagent Final concentration Amount DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) N/A 500 mL FBS 10% 50 mL Penicillin/Streptomycin 1% 5 mL (Continued on next page) 4 STAR Protocols 7, 104435, June 19, 2026

    Concentration Assay:

    Article Title: Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imaging.
    Article Snippet: MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) Gibco Cat#A1443001 2-Deoxy-D-Glucose (2-DG) Carl Roth Cat#CN96.2 Adenosine triphosphate (ATP) Carl Roth Cat#HN35.2 Cal-520 AM AAT Bioquest Cat#21130 Calcium chloride dihydrate (CaCl 2 ), CellPure Carl Roth Cat#HN04.2 DPBS, no calcium, no magnesium Gibco Cat#14190094 Fetal Bovine Serum (FBS) PAN-Biotech Cat#P30-3302 Fluo-3 AM AAT Bioquest Cat#21011 Fura Red AM AAT Bioquest Cat#21048 Glucose (stock 1.1 M) Gibco Cat#A2494001 HBSS, w/Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-32505 HBSS, w/o Ca 2+ and Mg 2+ PAN-Biotech Cat#P04-34500 IMDM (w/5.5 mM Glucose, w/o L-Glutamine) PAN-Biotech Cat#P04-20157 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldirch Cat#I0634-1MG L-Glutamine (stock 200 mM) PAN-Biotech Cat#P04-80100 Meso-Erythritol Thermo Scientific Car#A15813-14 Penicillin/Streptomycin PAN-Biotech Cat#P06-07100 Recombinant murine MCP-1 (CCL2) ImmunoTools Cat#12343383 Recombinant murine MIP-3β (CCL19) PeproTech Cat#250-27B Sodium Pyruvate (100 mM) PAN-Biotech Cat#P04-43100 Sucralose Biomol Cat#Cay31621-25 Thapsigargin Biomol Cat#Cay10522-1 UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Cat#15575020 β-Mercaptoethanol Carl Roth Cat#4227.3 Experimental models: Cell lines Mouse: J558L GM-CSF producing line Lutz et al., 7 , Burgdorf et al. 8 N/A Experimental models: Organisms/strain Mouse: C57BL76 (age: 8–12 weeks, male or female) Own breeding, N/A Software and algorithms BD FACS Diva BD Biosciences N/A Excel/Word, Office Standard 2019 Microsoft Office N/A FlowJo, Version 10 BD Biosciences N/A Graphpad Prism, Version 10 GraphPad Software https://www.graphpad.com/ Image J Schneider et al. 9 https://imagej.net/ij/ Other 15 mL/50 mL conical tubes Sarstedt Cat#62.554.502 BD LSR II, Flow Cytometer BD Biosciences N/A BD Symphony A5 BD Biosciences N/A Cell imaging plate, flat coverglass bottom Eppendorf Cat#0030741021 FACS tubes Sarstedt Cat#55.1579 Brightfield microscope: Nikon TS1000, Epifluorescence Microscopes: Nikon TI2, Olympus IX81 Nikon, Olympus N/A Medium containing physiological levels of glucose, pyruvate and glutamine Reagent Final concentration Amount DMEM (-D-Glucose, - L-Glutamine, - Sodium Pyruvate) N/A 500 mL FBS 10% 50 mL Penicillin/Streptomycin 1% 5 mL (Continued on next page) 4 STAR Protocols 7, 104435, June 19, 2026



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry

    doi: 10.1016/j.xpro.2022.101356

    Figure Lengend Snippet:

    Article Snippet: FACS tubes , Sarstedt , Cat# 55.1579.

    Techniques: Diagnostic Assay, Transplantation Assay, Recombinant, Saline, Staining, Blocking Assay, Red Blood Cell Lysis, Software, Flow Cytometry, Sterility